primary antibodies against f actin  (Novus Biologicals)

Journal: Viruses

Article Title: Impact of Dextran-Sodium-Sulfate-Induced Enteritis on Murine Cytomegalovirus Reactivation

doi: 10.3390/v14122595

Figure Lengend Snippet: Exploration of MCMV reactivation in the gut of mice. ( A )—Proportion of mice for which murine cytomegalovirus (MCMV) DNA was detected in small intestine specimens by PCR; positive mice are shown in black and negative ones in grey. ( B )—For colon specimens. ( C )—Immunohistochemistry at D37 (day of euthanasia) on gut specimens of a mouse of group 3 (upper part) or of group 4 (lower part). Staining corresponds to positive zones marked by horseradish peroxidase and revealed by diaminobenzidine substrate. The negative control was performed by omitting the primary antibody. The positive control was performed by using anti-alpha smooth muscle actin (anti-αSMA) as the primary antibody. The presence of a replicative virus was looked for by using an anti-immediate early (IE) 1 protein of MCMV as primary antibody. The scale bar on images corresponds to 50 µm. Photographs were taken at 20× objective in Zeiss Axioimager Apotome 3 optical microscope with a Zeiss quadriCCD Axiocam camera.

Article Snippet: Anti-m123/IE1 was diluted 1:500 in PBS and incubated on sections for 1 h at 37 °C f. Primary monoclonal antibody against alpha smooth muscle actin (anti-αSMA) (IgG2a kappa, Abcam, Cambridge, UK) was diluted 1:200 in PBS and incubated for 1 h at 37 °C for actin labelling as positive control.

Techniques: Immunohistochemistry, Staining, Negative Control, Positive Control, Microscopy